IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

The precursor of a psychrophilic α-amylase: structural characterization and insights into cold adaptation
Claverie, P.; Vigano, C.; Ruysschaert, J.-M.; Gerday, C.; Feller, G. (2003). The precursor of a psychrophilic α-amylase: structural characterization and insights into cold adaptation. Biochimica et Biophysica Acta-Proteins and Proteomics 1649(2): 119-122. dx.doi.org/10.1016/S1570-9639(03)00184-5
In: Biochimica et Biophysica Acta-Proteins and Proteomics. ELSEVIER SCIENCE BV: Amsterdam. ISSN 1570-9639, more
Peer reviewed article  

Available in  Authors 

Keyword
    Marine
Author keywords
    alpha-amylase; psychrophile; autotransporter; microcalorimetry; infraredspectroscopy

Authors  Top 
  • Claverie, P.
  • Vigano, C.
  • Ruysschaert, J.-M.

Abstract
    The α-amylase precursor from the bacterium Pseudoalteromonas haloplanktis possesses a propeptide at the C-terminus possibly responsible for outer membrane translocation. Unlike the predicted β-barrel of autotransporters, this C-terminal propeptide displays a noticeable α-helix content. It is connected to the enzyme by a disordered linker and has no significant interaction with the catalytic domain. The microcalorimetric pattern of the precursor also demonstrates that the stability of protein domains may evolve differently.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors