IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Primary cultures from the marine sponge Xestospongia muta (Petrosiidae, Haplosclerida)
Richelle-Maurer, E.; Gomez, R.; Braekman, J.C.; Van de Vyver, G.; Van Soest, R.W.M.; Devijver, C. (2003). Primary cultures from the marine sponge Xestospongia muta (Petrosiidae, Haplosclerida). Journal of biotechnology 100(2): 169-176. dx.doi.org/10.1016/S0168-1656(02)00251-1
In: Journal of biotechnology. Elsevier. ISSN 0168-1656, more
Peer reviewed article  

Available in Authors 

Keyword
    Marine
Author keywords
    Xestospongia muta; Haplosclerida; cell culture; skeleton; straight-chainacetylenic compounds

Authors  Top 
  • Richelle-Maurer, E.
  • Gomez, R.
  • Braekman, J.C., more
  • Van de Vyver, G.
  • Van Soest, R.W.M., more
  • Devijver, C.

Abstract
    In the context of the investigations on the origin and in vitro production of bioactive compounds, primary cultures were developed from ectosomal and choanosomal cell suspensions from the sponge Xestospongia muta. Dissociated cells aggregated and reorganized into a striking reticulated network of cells, typical for X. muta. Moreover, in some cultures an isotropic reticulation of small spicules, very similar to that found in the ectosome of adult sponges, was observed. Phytohaemagglutinin promoted aggregation and the reorganization of the cells. HPLC analyses revealed that straight-chain acetylenic compounds were recovered from short-term cultures and that they were synthesized during culture. Heterotrophic bacteria were assumed to be involved in the process. Together our results established that X. muta would be an excellent experimental model to study, in laboratory conditions, the differentiation of the skeleton and the in vitro biosynthesis of straight-chain acetylenic compounds.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors