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Characterization of Pseudoalteromonas citrea and P. nigrifaciens isolated from different ecological habitats based on REP-PCR genomic fingerprints
Ivanova, E.P.; Matte, G.R.; Matte, M.H.; Coenye, T.; Huq, A.; Colwell, R.R. (2002). Characterization of Pseudoalteromonas citrea and P. nigrifaciens isolated from different ecological habitats based on REP-PCR genomic fingerprints. Syst. Appl. Microbiol. 25(2): 275-283. dx.doi.org/10.1078/0723-2020-00103
In: Systematic and Applied Microbiology. Elsevier: Jena. ISSN 0723-2020, more
Peer reviewed article  

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Keywords
    Pseudoalteromonas Gauthier, Gauthier & Christen, 1995 [WoRMS]; Marine
Author keywords
    Pseudoalteromonas; biogeography; REP; ERIC; BOX-PCR

Authors  Top 
  • Ivanova, E.P.
  • Matte, G.R.
  • Matte, M.H.
  • Coenye, T., more
  • Huq, A.
  • Colwell, R.R.

Abstract
    DNA primers corresponding to conserved repetitive interspersed genomic motifs and PCR were used to show that REP, ERIC and BOX-like DNA sequences are present in marine, oxidative, Gram-negative Pseudoalteromonas strains. REP, ERIC and BOX-PCR were used for rapid molecular characterization of both the type species of the genus and environmental strains isolated from samples collected in different geographical areas. PCR-generated genomic fingerprint patterns were found to be both complex and strain specific. Analysis of the genotypic structure of phenotypically diverse P. citrea revealed a geographic clustering of Far Eastern brown-pigmented, agar-digesting strains of this species. Marine isolates of P. nigrifaciens with 67–70% DNA relatedness generated genomic patterns different from those of the type strain and formed a separate cluster. It is concluded that REP, ERIC and BOX-PCR are effective in generating strain specific patterns that can be used to elucidate geographic distribution, with these genomic patterns providing a valuable biogeographic criterion.

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