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Formation of angiotensin-(1-7) from angiotensin II by the venom of Conus geographus
Le, M.T.; Vanderheyden, P.M.L.; Baggerman, G.; Vanden Broeck, J.; Vauquelin, G. (2002). Formation of angiotensin-(1-7) from angiotensin II by the venom of Conus geographus. Regulatory Peptides 105(2): 101-108. dx.doi.org/10.1016/S0167-0115(02)00005-8
In: Regulatory Peptides. Elsevier: Amsterdam. ISSN 0167-0115, more
Peer reviewed article  

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Keywords
    Conus geographus Linnaeus, 1758 [WoRMS]; Marine
Author keywords
    angiotensin II; CHO-AT(1) cells; Conus geographus; radioligand binding;mass spectrometry

Authors  Top 
  • Le, M.T.
  • Vanderheyden, P.M.L.
  • Baggerman, G.
  • Vanden Broeck, J.
  • Vauquelin, G.

Abstract
    The binding of [3H]angiotensin II to AT1 receptors on Chinese Hamster Ovary cells expressing the human AT1 receptor (CHO-AT1 cells) is potently inhibited by venoms of the marine snails Conus geographus and C. betulinus. On the other hand, the binding of the nonpeptide AT1 receptor-selective antagonist [3H]candesartan is not affected but competition binding curves of angiotensin II and the peptide antagonist [Sar1,Ile8]angiotensin II (sarile) are shifted to the right. These effects resulted from the breakdown of angiotensin II into smaller fragments that do not bind to the AT1 receptor. In this context, angiotensin-(1–7) is the most prominent fragment and angiotensin-(1–4) and angiotensin-(1–5) are also formed but to a lesser extent. The molecular weight of the involved peptidases exceeds 50 kDa, as determined by gel chromatography and ultrafitration.

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