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Molecular phylogeny of mangroves: 9. Molecular marker assisted intra-specific variation and species relationships in the Indian mangrove tribe Rhizophoreae
Lakshmi, M.; Parani, M.; Parida, A. (2002). Molecular phylogeny of mangroves: 9. Molecular marker assisted intra-specific variation and species relationships in the Indian mangrove tribe Rhizophoreae. Aquat. Bot. 74(3): 201-217. dx.doi.org/10.1016/s0304-3770(02)00105-5
In: Aquatic Botany. Elsevier Science: Tokyo; Oxford; New York; London; Amsterdam. ISSN 0304-3770, more
Peer reviewed article  

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Keywords
    Biomarkers; Chemotaxonomy; Conservation; DNA; Genetic diversity; Hybrids; Intraspecific relationships; Mangroves; Phylogeny; Bruguiera cylindrica (Linnaeus) Blume [WoRMS]; Bruguiera gymnorrhiza (L.) Lamk. [WoRMS]; Bruguiera parviflora Wight & Arnold ex Griffith [WoRMS]; Ceriops decandra (Griff.) Ding Hou [WoRMS]; Ceriops tagal (Perr.) C.B. Robinson [WoRMS]; Rhizophora apiculata Bl. [WoRMS]; Rhizophora mucronata Lamk. [WoRMS]; Rhizophora stylosa Griff. [WoRMS]; ISW, India [Marine Regions]; ISW, India, Andaman I. [Marine Regions]; ISW, India, Nicobar I. [Marine Regions]; Brackish water

Authors  Top 
  • Lakshmi, M.
  • Parani, M.
  • Parida, A.

Abstract
    Molecular phylogeny and genetic diversity in all the nine species (Rhizophora mucronata, R. apiculata, R. stylosa, Bruguira cylindrical, B. parviflora,B. gymnorriza, Ceriops tagal and C. decandra) and a natural Rhizophora hybrid, of the mangrove Rhizophoraceae, occurring in the Indian sub-continent were analysed using random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) and RFLP of polymerase chain reaction amplified chloroplast genes (PCR-RFLP) as markers. Intra-specific variability as revealed by RAPDs were low in all the analysed species. Inter-specific RFLP analysis revealed species-specific profiles in some probe-enzyme combinations. The rDNA repeat units, as flanked by the Hind III restriction sites was found to be very conserved within each genus and three different rDNA repeat units were observed among the four genera. Generic differences in PCR-RFLP were observed only in rbcL and trnS-psbC gene regions. Species differences were observed in Rhizophora in the PCR-RFLP of trnS-psbC and trnL-UAA with Hae III and Taq I, respectively. Rhizophora mucronata was found to be the chloroplast donor for the natural inter-specific hybrid. A dendrogram based on the data sets from all the three marker systems revealed that the four genera segregated into three species groups.

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