Suitability of the Fluorescence Monitoring System (FMS, Hansatech) for measurement of photosynthetic characteristics in algae
Mouget, J.-L.; Tremblin, G. (2002). Suitability of the Fluorescence Monitoring System (FMS, Hansatech) for measurement of photosynthetic characteristics in algae. Aquat. Bot. 74(3): 219-231. http://dx.doi.org/10.1016/s0304-3770(02)00104-3
In: Aquatic Botany. Elsevier Science: Tokyo; Oxford; New York; London; Amsterdam. ISSN 0304-3770; e-ISSN 1879-1522, more
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| Keywords |
Algae
Analytical techniques > Spectroscopic techniques > Fluorescence spectroscopy
Chemical compounds > Organic compounds > Carbohydrates > Glycosides > Pigments > Photosynthetic pigments
Chemical reactions > Photochemical reactions > Photosynthesis
Equipment > Instruments
Equipment > Measuring devices
Organic compounds > Carbohydrates > Glycosides > Pigments > Photosynthetic pigments > Chlorophylls
Fucus serratus Linnaeus, 1753 [WoRMS]; Palmaria palmata (Linnaeus) F.Weber & D.Mohr, 1805 [WoRMS]; Ulva Linnaeus, 1753 [WoRMS]
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| Author keywords |
chlorophyll fluorescence; macroalgae; microalgae; modulationfluorometer; quenching analysis |
| Authors | | Top |
- Mouget, J.-L.
- Tremblin, G.
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| Abstract |
A pulse-modulated fluorescence measuring system, originally developed for higher plant studies, the Fluorescence Monitoring System (FMS; Hansatech), was tested to study photosynthesis in algae. The main technical features of the FMS are described, and applications of the saturating pulse fluorescence method are presented on micro- and macroalgae. Some limitations of the apparatus for measuring fluorescence characteristics of microalgae are discussed. Chlorophyll a fluorescence quenching analyses were performed on macroalgae from different taxonomic groups (Ulva sp., Palmaria palmata, Fucus serratus), and the results of the fluorescence measurements are presented and discussed, in relation to the macroalgae characteristics. The FMS and the saturating pulse method may be successfully used with green and red algae, in contrast to brown algae, with which F'm values higher than Fm were observed at low actinic light (<100µmol m-2s-1). This unconventional result is detected by error trapping routines and prevents employing the entire FMS potentiality. This point is discussed taking into account FMS specifications and limits. |
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