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Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (Artemia spp.) used for prophylactic chemotherapy of fish
Nelis, H.; Léger, P.; Sorgeloos, P.; De Leenheer, A.P. (1991). Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (Artemia spp.) used for prophylactic chemotherapy of fish. Antimicrob. Agents Chemother. 35(12): 2486-2489
In: Antimicrobial agents and chemotherapy. American Society for Microbiology: Washington, D.C.. ISSN 0066-4804, more
Peer reviewed article  

Also published as
  • Nelis, H.; Léger, P.; Sorgeloos, P.; De Leenheer, A.P. (1991). Liquid chromatographic determination of efficacy of incorporation of trimethoprim and sulfamethoxazole in brine shrimp (Artemia spp.) used for prophylactic chemotherapy of fish, in: IZWO Coll. Rep. 21(1991). IZWO Collected Reprints, 21: pp. chapter 37, more

Available in Authors 

Keywords
    Aquaculture techniques; Fish culture; Infectious diseases; Medicine; Artemia Leach, 1819 [WoRMS]; Brackish water; Fresh water

Authors  Top 
  • Nelis, H.
  • Léger, P.
  • Sorgeloos, P., more
  • De Leenheer, A.P.

Abstract
    The brine shrimp Artemia , an excellent live food source in aquaculture, has been studied as a carrier to deliver selected chemotherapeutic agents to fish for prophylactic treatment of infectious diseases. To monitor the efficiency of incorporation of trimethoprim and sulfamethoxazole in Artemia franciscana, a sensitive and specific analytical method was developed. It is based on homogenization of Artemia nauplii in methanol, extraction of lipids with hexane, solid-phase cleanup on C18 cartridges, and reversed-phase liquid chromatography with detection at 210 nm. The method is sensitive (detection limit, on the order of 3 µg/g with a sample quantity of 30 mg [dry weight]) and reproducible (coefficients of variation, 2.2 and 1.8% for trimethoprim and sulfamethoxazole at levels of 79.6 and 257 µg/g of body weight, respectively). Preliminary quantitative data indicated excellent uptake and persistence of both therapeutic agents in A. franciscana, with levels of 115 µg/g for trimethoprim and 277 µg/g for sulfamethoxazole.

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