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Molecular variability of Chrysoperla Steinmann, 1964 (Neuroptera: Chrysopidae) inhabiting western Saudi Arabia
Sayed, S.M.; Amer, S.A. (2015). Molecular variability of Chrysoperla Steinmann, 1964 (Neuroptera: Chrysopidae) inhabiting western Saudi Arabia. Pan-Pac. Entomol. 91(2): 101-107. https://hdl.handle.net/10.3956/2015-91.2.101
In: The Pan-Pacific Entomologist. Pacific Coast Entomological Society: San Francisco,. ISSN 0031-0603; e-ISSN 2162-0237, more
Peer reviewed article  

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Author keywords
    mitochondrial DNA, biodiversity, green lacewing

Authors  Top 
  • Sayed, S.M.
  • Amer, S.A.

Abstract
    The large ribosomal RNA subunit (16S rRNA) and cytochrome C subunit 3 (CO3) genes have been partially amplified and sequenced for the species of Chrysoperla Steinmann, 1964 (Neuroptera: Chrysopidae) inhabiting the western part of Saudi Arabia to examine their molecular variability. Insect samples were collected from three distant localities, which were Tabouk in the north, Jeddah in the middle and Jazan in the south of the country. The concatenated sequences (904 bp) have been manipulated by three analytical methods (maximum-parsimony, neighbor-joining and maximum-likelihood) producing one fixed tree. The tree topology divided, with strong statistical supports, Saudi Arabian Chrysoperla into two clusters. Jeddah and Tabouk samples clustered (BP = 100%) with both Chrysoperla carnea (Stephens, 1836) and Chrysoperla nipponensis (Okamoto, 1914), while Jazan samples grouped (BP = 100%) with Dichochrysa tacta (Navás, 1921). Both pairwise genetic distances obtained from the concatenated data and amino acid substitution in CO3 gene supported this division. It could be concluded that Saudi Arabian Chrysoperla constitutes a species complex and requires further molecular investigation.

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