Immunolocalization of catecholamine enzymes, serotonin, dopamine and L-dopa in the brain of Dicentrarchus labrax (Teleostei)
Batten, T.F.C.; Berry, P.A.; Maqbool, A.; Moons, L.; Vandesande, F. (1993). Immunolocalization of catecholamine enzymes, serotonin, dopamine and L-dopa in the brain of Dicentrarchus labrax (Teleostei). Brain Research Bulletin 31(3-4): 233-252. https://dx.doi.org/10.1016/0361-9230(93)90214-V
In: Brain Research Bulletin. PERGAMON-ELSEVIER SCIENCE LTD: Oxford. ISSN 0361-9230; e-ISSN 1873-2747, more
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CATECHOLAMINE; DOPAMINE; 5-HYDROXYTRYPTAMINE; L-DOPA; SYNTHESIZINGENZYME; IMMUNOHISTOCHEMISTRY BRAIN; TELEOST FISH |
| Authors | | Top |
- Batten, T.F.C.
- Berry, P.A.
- Maqbool, A.
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| Abstract |
Antisera to serotonin (5-HT), dopamine, and L-dopa, and to the catecholamine synthesizing enzymes, tyrosine hydroxylase (TH), dopamine β-hydroxylase (DBH), and phenylethanolamine N-methyl transferase (PNMT), were used to localize monoamine containing neurones in the brain of Dicentrarchus labrax (sea bass). In the brain stem, 5-HT-immunoreactive (ir) neurones were recognized in the ventrolateral medulla, vagal motor area, medullary, and mesencephalic raphe nuclei and in the dorsolateral isthmal tegmentum. In the hypothalamus, liquor-contacting 5-HT neurones were seen in various regions of the paraventricular organ. Virtually all regions of the brain contained a dense innervation by 5-HT fibres and terminals. DBH-ir neurones were restricted to three brain stem areas: the locus coeruleus, the area postrema, and the reticular formation of the lower medulla. Neurones in these three groups also displayed TH-ir, and in the latter area, PNMT-ir in addition. In the locus coeruleus and area postrema, TH-ir neurones outnumbered DBH-ir neurones, an observation substantiated by the presence of dopamine-ir neurones. In the forebrain, dopamine- and TH-ir neurones were found in the olfactory bulb, ventral/central telencephalon, periventricular preoptic, and suprachiasmatic areas, dorsolateral and ventromedial thalamus, and posterior tuberal nucleus. In the paraventricular organ, the distribution and morphology of dopamine-ir neurones was similar to that observed with anti-5-HT, but the vast majority of cells were not TH-ir, suggesting accumulation of dopamine by uptake from the ventricle, rather than by synthesis. L-dopa-ir neurones were found only in the central telencephalon, preoptic recess, and dorsolateral thalamus. Fibres and terminals immunoreactive for dopamine, TH, and DBH showed a broadly similar distribution. The results are discussed in relation to the monoaminergic systems previously reported in other teleostean species and the mammalian brain. |
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