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Anti-haemostatic compounds from the vampire snail Cumia reticulata: molecular cloning and in-silico structure-function analysis
Modica, M.V.; Sánchez, J.R.; Pasquadibisceglie, A.; Oliverio, M.; Mariottini, P.; Cervelli, M. (2018). Anti-haemostatic compounds from the vampire snail Cumia reticulata: molecular cloning and in-silico structure-function analysis. Computational Biology and Chemistry 75: 168-177.
In: Computational Biology and Chemistry. Elsevier SCI Ltd: Oxford. ISSN 1476-9271; e-ISSN 1476-928X, more
Peer reviewed article  

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Author keywords
    Mollusc; Anti-haemostatic compounds; Protein modelling

Authors  Top 
  • Modica, M.V.
  • Sánchez, J.R.
  • Pasquadibisceglie, A.
  • Oliverio, M.
  • Mariottini, P.
  • Cervelli, M.

    Blood-feeding animals are known for their ability to produce bioactive compounds to impair haemostasis and suppress pain perception in the host. These compounds are extremely appealing for pharmacological development since they are generally very effective and specific for their molecular target. A preliminary RNA-Seq based characterization of the secretion from salivary and mid-oesophageal tissues of the vampire snail Cumia reticulata, revealed a complex mixture of feeding-related transcripts with potential anaesthetic and anticoagulant action. Based on the cloned full-length mRNAs, it was possible to verify the sequence of five genes encoding haematophagy-related products. The in silico modelled three-dimensional structure of each translational product was analysed to gain information on their potential biochemical activity. We have hereby validated and further investigated the assembled transcripts presumably involved in the antihaemostatic action, to improve our comprehensive understanding of this subset of the feeding secretion. The studied proteins included both inhibitors of primary haemostasis such as the vWFA domain-containing proteins, and compounds targeting different steps of the coagulation cascade, as e.g. the Turripeptide-like/protease inhibitor, the TFPI-like multiple Kunitz-type protease inhibitors, the Meprin-like metalloproteases and the Astacin/ShKT-like domain-containing proteins. All these molecules showed promising potential for pharmacological development.

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