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Protocols for macroalgae research
Charrier, B.; Wichard, T.; Reddy, C.R.K. (Ed.) (2018). Protocols for macroalgae research. CRC Press Taylor & Francis Group: Boca Raton. ISBN 978-1498796422. 491 pp.

Available in  Authors 
    VLIZ: Botany [101015]

Keywords
    Chemical compounds > Organic compounds > Proteins
    Chemistry > Biochemistry
    Exploitation
    Flora > Weeds > Marine organisms > Seaweeds
    Metabolism
    Preservation
    Protocols
    Technology > Biotechnology
    Marine

Authors  Top 
  • Charrier, B., editor
  • Wichard, T., editor
  • Reddy, C.R.K., editor

Content
  • Buck, B.H.; Grote, B. (2018). Seaweed in high-energy environments: Protocol to move Saccharina cultivation offshore, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 3-36, more
  • Forbord, S.; Steinhovden, K.B.; Rød, K.K.; Handå, A.; Skjermo, J. (2018). Cultivation protocol for Saccharina latissima, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 37-59, more
  • Bartsch, I. (2018). Derivation of clonal stock cultures and hybridization of kelps: A tool for strain preservation and breeding programs, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 61-78, more
  • Day, J. (2018). Cryopreservation of macroalgae, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 79-94, more
  • Aires, T.; Muyzer, G.; Serrão, E.A.; Engelen, A. (2018). Unraveling seaweeds bacteriomes: From field site to computer screen , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 95-113, more
  • Leal, P.P.; Roleda, M.Y. (2018). Heavy metal ecotoxicity on the early life history stages of macroalgae , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 115-127, more
  • Gupta, V.; Reddy, C.R.K. (2018). A simple protocol for a rapid and consistent production of a large number of viable protoplasts from the Ulvophycean species , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 129-138, more
  • Kessler, R.W.; Alsufyani, T.; Wichard, T. (2018). Purification of sporulation and swarming inhibitors from Ulva: Application in algal life-cycle controlling , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 139-157, more
  • Califano, G.; Wichard, T. (2018). Preparation of axenic cultures in Ulva (Chlorophyta), in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 159-171, more
  • Contreras-Porcia, L.; Araya, M.; Garrido-Ramírez, E.; Bulboa, C.; Remonsellez, J.P.; Zapata, J.; Espinoza, C.; Rivas, J. (2018). Biochar production from seaweeds, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 175-185, more
  • Graiff, A.; Ruth, W.; Karsten, U. (2018). Identification and quantification of laminarins in brown algae , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 187-198, more
  • Huijgen, W.J.J.; Cobussen-Pool, E.M.; van Egmond, B.F.; van Hal, J.W. (2018). Determination of carbohydrate composition of macroalgae , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 199-210, more
  • Safina, C.; van Leeuwen, J.; Telleman, Y.; Engelen-Smit, N.; van den Broek, L.; Harmsen, P. (2018). Quantification of proteins in seaweeds, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 211-224, more
  • Matsuura, T.; Mori, I.C.; Ikeda, Y.; Hirayama, T.; Mikami, K. (2018). Comprehensive phytohormone quantification in the red alga Pyropia yezoensis by liquid chromatography–mass spectrometry , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 223-236, more
  • Hou, X.; Neerup, R.; Bjerre, A.-B. (2018). Total phenolic content and antioxidant capacity analysis of seaweed biomass , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 236-248, more
  • Beattie, S.W.; Morançais, M.; Déléris, P.; Fleurence, J.; Dumay, J. (2018). Extraction of phycocyanin and phycoerythrin pigments, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 249-266, more
  • Kumar, M.; Contreras-Porcia, L.; Kumar, N.M.; Ralph, P.J. (2018). Quantification and localization of reactive oxygen species in marine macrophytes , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 266-277, more
  • Kuhlisch, C.; Califano, G.; Wichard, T.; Pohnert, G. (2018). Metabolomics of intra- and extracellular metabolites from micro- and macroalgae using GC–MS and LC–MS , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 279-300, more
  • Weinberger, F. (2018). Preparative extraction of exometabolites from seaweed surfaces, in: Charrier, B. et al. Protocols for macroalgae research. pp. 300-308, more
  • Cirri, E.; Pohnert, G. (2018). Disruption-free solid-phase extraction of surface metabolites from macroalgae , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 308-319, more
  • Siméon, A.; Duffieux, D.; Hervé, C.; Le Panse, S.; Knox, P.; Torode, T.A. (2018). The immunodetection and in situ imaging of cell-wall polysaccharides in brown algae , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 323-334, more
  • Torode, T.A.; Linardic, M.; Kaplan, J.L.; Braybrook, S. (2018). Atomic force microscopy based analysis of cell-wall elasticity in macroalgae , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 335-347, more
  • Rabillé, H.; Billoud, B.; Rolland, E.; Charrier, B. (2018). Dynamic and microscale mapping of cell growth: Case of Ectocarpus filament cells, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 349-364, more
  • Rabillé, H.; Koutalianou, M.; Charrier, B.; Katsaros, C. (2018). Actin fluorescent staining in the filamentous brown alga Ectocarpus siliculosus , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 365-379, more
  • Nagasato, C.; Katsaros, C.; Motomura, T. (2018). Cryofixation of brown algae for transmission electron microscopy , in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 381-389, more
  • Raimundo, S.C.; Domozych, D.S. (2018). Probing the subcellular topography of seaweeds: Transmission electron microscopy, immunocytochemistry, and correlative light microscopy, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 391-410, more
  • Kaleb, S.; Alongi, G.; Falace, A. (2018). Coralline algae preparation for scanning electron microscopy and optical microscopy, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 411-428, more
  • Heinrich, S. (2018). Extraction of high quality RNA from brown alga for transcriptomic analysis, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 429-439, more
  • Saint-Marcoux, D; Langdale, J.A. (2018). Induction of sexual reproduction in Spirogyra cultures for laser capture microdissection of gametes and zygotes, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 441-452, more
  • Groisillier, A. (2018). Cloning and expression strategies for the postgenomic analysis of brown algae, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 453-468, more
  • Boesger, J.; Kwantes, M.; Wichard, T. (2018). Polyethylene glycol-mediated transformation in the green macroalga Ulva mutabilis (Chlorophyta): A forward genetics approach, in: Charrier, B. et al. (Ed.) Protocols for macroalgae research. pp. 469-483, more

Abstract
    Protocols for Macroalgae Research describes state-of-the-art approaches and applications in seaweed research. The book is comprised of 31 protocols in all and provides critical information regarding the cultivation and preservation of seaweeds, their chemical composition, as well as their cellular and molecular characterization. This type of research is critical and timely, as the last few decades have witnessed remarkable advancements in phycological research worldwide. Consideration of algal resources for food, fuel, chemicals, feed, and fertilizer has furthered the development of diverse laboratory techniques that not only advance their utilization substantially, but also provided new insights in understanding the molecular basis of their form and function. This recent progress has enabled researchers to determine and quantify various metabolites and organic macromolecules such as carbohydrates, proteins, and lipids. Protocols for Macroalgae Research thus uses macroalgae as a model organism for addressing fundamental research problems. No recent comprehensive literature of this format exists on this topic. As such, this state-of-the-art protocol text both develops and standardizes the various macroalgae approaches located within the field. It does so by featuring a wide-range of protocols from invited experts; each protocol features a brief introductory overview, an inventory of materials and reagents, and step-by-step experimental procedures, which include helpful notes offering care and cautions. This protocol book is an invaluable reference for beginning and preexisting researchers within the field of macroalgae.

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