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Identification of N-acyl homoserine lactone-degrading bacteria isolated from rainbow trout (Oncorhynchus mykiss)
Delshad, S.T.; Soltanian, S.; Sharifiyazdi, H.; Haghkhah, M.; Bossier, P. (2018). Identification of N-acyl homoserine lactone-degrading bacteria isolated from rainbow trout (Oncorhynchus mykiss). J. Appl. Microbiol. 125(2): 356-369. https://hdl.handle.net/10.1111/jam.13891
In: Journal of Applied Microbiology. Blackwell Science: Oxford. ISSN 1364-5072; e-ISSN 1365-2672, more
Peer reviewed article  

Available in  Authors 

Author keywords
    aquaculture; biocontrol; degradation; identification; quorum sensing

Authors  Top 
  • Delshad, S.T., more
  • Soltanian, S.
  • Sharifiyazdi, H.
  • Haghkhah, M.
  • Bossier, P., more

Abstract
    Aims

    A variety of pathogens use quorum sensing (QS) to control the expression of their virulence factors. QS interference has hence been proposed as a promising antivirulence strategy. The specific aim of this study was to isolate bacteria from trout tissue able to degrade N‐acyl homoserine lactones (AHL), a QS molecule family.

    Methods and Results

    In total 132 isolates were screened for AHL degradation using Chromobacterium violaceum CV026 as a biosensor. Twenty‐four quorum‐quenching (QQ) isolates were identified biochemically and characterized using 16S rDNA sequencing. They belong to Bacillus, Enterobacter, Citrobacter, Acinetobacter, Agrobacterium, Pseudomonas and Stentrophomonas genera. Four Bacillus spp. showed the highest and fastest QQ activity. AHL degradation proved to be enzymatic in most isolates (except for Stentrophomonas spp. and Pseudomonas sp.) as QQ activity could be destroyed by heat and/or proteinase K treatments. All QQ activity proved to be cell‐bound except for Pseudomonas sp., where it could be detected in the supernatant. The results of aiiA gene homology analysis revealed the presence of aiiA gene encoding AHL lactonase in all examined isolates except Pseudomonas syringae and Enterobacter cloacae. The HXHXDH motif conserved in all AHL lactonases and considered to be essential for AHL degradation was detected in all AiiAs after sequence alignment.

    Conclusions

    Some known and novel QQ bacteria were isolated from trouts and characterized in terms of enzymatic or nonenzymatic AHL degradation activity and their extracellular or intracellular location. In addition, an aiiA gene and its HXHXDH motif were detected in most isolates.

    Significance and Impact of the Study

    We could isolate and identify some novel QQ bacteria including Enterobacter hormaechei, Acinetobacter radioresistens and Citrobacter gillenii. The aiiA gene was detected for the first time in these strains as well as in Stenotrophomonas maltophilia. Our QQ isolates could be used for biocontrol of bacterial infections in aquaculture.


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