On the hemagglutinins contained in agar seaweed (Gelidium): III. Purification and its biological specific character of a Gelidium amansii hemagglutinin
Yamada, N.; Emori, M.; Furutani, S.; Amagai, Y.; Muramatsu, K. (1999). On the hemagglutinins contained in agar seaweed (Gelidium): III. Purification and its biological specific character of a Gelidium amansii hemagglutinin. Bull. Inst. Oceanic Res. & Develop., Tokai Univ. 20: 85-92
In: Bulletin of Institute of Oceanic Research and Development. Tokai University. Tokai University. Institute of Oceanic Research and Development: Shimizu. ISSN 0289-680X, more
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| Authors | | Top |
- Yamada, N.
- Emori, M.
- Furutani, S.
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| Abstract |
We have already reported the experimental results of hemagglutinin yield and aggregative activity in samples obtained from 53 specimens of the domestic seaweed Gelidium amansii collected at various locations and at different sea depths. These results were obtained using the crude extracts of the organism. It would be helpful however, to conduct further experiments after purification and isolation of the extracts in order to clarify the above subject accurately.Since we were unable to find any previous reports on the purification and isolation of Gelidium amansii lectin, we isolated the lectin using chitin affinity chromatography. The results confirmed that the method was effective in isolating the lectin and that homogenization was not necessary to obtain crude extracts.The yield of Gelidium amansii lectin was about 17mg/100g in dry weight. Blood and sugar specificity was compared between lectins isolated from land plants, animals and seaweeds sold on the market.Regarding blood specificity, Gelidium amansii lectin strongly agglutinated untreated rabbit and rat erythrocytes. The results on sugar specificity showed that the lectin hardly recognized monosaccharides. Lactoferrin was the most reactive against the lectin among 14 kinds of glycoprotein. |
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