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Appearance stages of alginase in the juvenile abalone, Haliotis discus of hatchery seeds
Yamada, N.; Yasuda, A.; Yamazaki, Y.; Inaba, Y.; Satake, K.; Isida, T.; Washiyama, H. (1999). Appearance stages of alginase in the juvenile abalone, Haliotis discus of hatchery seeds. Bull. Inst. Oceanic Res. & Develop., Tokai Univ. 20: 93-98
In: Bulletin of Institute of Oceanic Research and Development. Tokai University. Tokai University. Institute of Oceanic Research and Development: Shimizu. ISSN 0289-680X, more
Peer reviewed article  

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  • Yamada, N.
  • Yasuda, A.
  • Yamazaki, Y.
  • Inaba, Y.
  • Satake, K.
  • Isida, T.
  • Washiyama, H.

Abstract
    It is well known that the abalones consume the brown algae, Phaeophyta, which contains alginic acid. Oshima (1931) reported that the large quantities of alginase were observed in the internal organs of adults. However, the size of the abalone at which alginase first appears has not yet been determined.The present experiments were thus conducted to determine the alginase content in hatchery seeds of abalone, in order to understand the optimum feeding manual for seeding production.Determination of alginase content was carried out by measuring the unsaturated uronide formation complated by sodium alginic acid.As a result, alginase was measured in larvae 35 days old 1.67 mm size. The alginase contents increased gradually thereafter.This suggests that brown algae should be supplied when the larvae grow to a length of about 2 mm, which could lead to an improvement in growth and survival rates.

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