IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Production and characterization of monoclonal antibodies against tilapia Oreochromis niloticus immunoglobulin
Al-Harbi, A.H.; Truax, R.; Thune, R.L. (2000). Production and characterization of monoclonal antibodies against tilapia Oreochromis niloticus immunoglobulin. Aquaculture 188: 219-227
In: Aquaculture. Elsevier: Amsterdam; London; New York; Oxford; Tokyo. ISSN 0044-8486, more
Peer reviewed article  

Available in Authors 

Keyword
    Marine

Authors  Top 
  • Al-Harbi, A.H.
  • Truax, R.
  • Thune, R.L.

Abstract
    Eight monoclonal antibodies (MAbs) against tilapia immunoglobulins (Ig) were produced and characterized. All of the MAbs are functional for use in enzyme-linked immunosorbent assays (ELISAs) and immunoblotting applications. Characterization of the MAbs for mouse Ig class and subclass indicate that four were IgG 2b, two were IgG 2a, one was IgG 3, and one was IgM K class. Western blot analysis of the eight hybridomas with purified Nile tilapia Ig indicated that all eight MAbs were heavy chain specific and further analysis indicated that all eight MAbs react with tilapia Ig H chain and cross-reactive with other tilapia species and their hybrids.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors