IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability
Peruzzi, S.; Chatain, B. (2000). Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability. Aquac. Res. 189: 23-37
In: Aquaculture Research. Blackwell: Oxford. ISSN 1355-557X, more
Peer reviewed article  

Available in Authors 

Keyword
    Marine

Authors  Top 
  • Peruzzi, S.
  • Chatain, B.

Abstract
    The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0-1°C for 15-20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm-2) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was carried out by microsatellite marker loci analysis. This work highlights the degree of variation in individual responses of selected broodstock to these agents. Finally, some preliminary results on heterologous fertilisation in sea bass with potential applications for gynogenetic studies are also provided.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors