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Differential expression of Atlantic salmon thyrotropin ß subunit mRNA and its cDNA sequence
Martin, S.A.M.; Wallner, W.; Youngston, A.F.; Smith, T. (1999). Differential expression of Atlantic salmon thyrotropin ß subunit mRNA and its cDNA sequence. J. Fish Biol. 54: 757-766
In: Journal of Fish Biology. Fisheries Society of the British Isles: London,New York,. ISSN 0022-1112, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Martin, S.A.M.
  • Wallner, W.
  • Youngston, A.F.
  • Smith, T.

Abstract
    To study the regulation of the thyroid system, an Atlantic salmon Salmo salar cDNA clone was isolated for thyroid stimulating hormone (TSH) ß subunit gene. A cDNA (866 bp) was isolated from an adult Atlantic salmon pituitary cDNA library, this clone was sequenced and shown to be highly conserved when compared to other teleost ß TSH subunit sequences. The cDNA was used as a probe for Northern blot analysis of total pituitary RNA from the different life cycle stages of Atlantic salmon. Northern blot analysis demonstrated that ß TSH mRNA is expressed at all life cycle stages studied, including parr, smolt, immature fish at sea and sexually mature male fish. Densitometry of Northern blots showed that sexually mature male salmon had low levels of salmon ß TSH mRNA compared to non-mature fish. Stunts, fish performing poorly in salt water, were shown to have elevated levels of ß TSH mRNA when compared to healthy fish.

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