Pfiesteria shumwayae (Pfiesteriaceae) in New Zealand
In: New Zealand Journal of Marine and Freshwater Research. Royal Society of New Zealand: Wellington. ISSN 0028-8330; e-ISSN 1175-8805, more
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Authors | | Top |
- Rhodes, L.L.
- Burkholder, J.M.
- Glasgow, H.B.
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- Rublee, P.A.
- Allen, C.
- Adamson, J.E.
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Abstract |
Pfiesteria shumwayae Steidinger et Burkholder is now known to be present in New Zealand and occurs in estuaries around the country. The presence of Pfiesteria was initially determined by a polymerase chain reaction (PCR)-based detection assay, using oligonucleotide primers targeted at ribosomal DNA extracted from estuarine water and sediments. Presence was confirmed by isolation from fresh sediments in the presence of fish (Oreochromis mossambicus), followed by identification by scanning electron microscopy. The New Zealand isolates of P. shumwayae were ichthyotoxic in bioassays, but there is no historic evidence of fish kills in New Zealand associated with the dinoflagellate. |
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