Physiological responses of Gracilariopsis longissima (S.G. Gmelin) Steentoft, L.M. Irvine and Farnham (Rhodophyceae) to sub-lethal copper concentrations
Brown, M.T.; Newman, J.E. (2003). Physiological responses of Gracilariopsis longissima (S.G. Gmelin) Steentoft, L.M. Irvine and Farnham (Rhodophyceae) to sub-lethal copper concentrations. Aquat. Toxicol. 64(2): 201-213
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514, more
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| Keywords |
Chemical elements > Metals > Transition elements > Heavy metals > Copper
Chemical elements > Nonmetals > Atmospheric gases > Oxygen
Defects > Leaks
Ions
Luminescence > Fluorescence
Organic compounds > Carbohydrates > Glycosides > Pigments > Photosynthetic pigments > Chlorophylls
Population functions > Growth
Rhodophyceae [WoRMS]
Marine/Coastal |
| Authors | | Top |
- Brown, M.T., correspondent
- Newman, J.E.
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| Abstract |
Through a series of comparative experiments the relative effects of copper (Cu) exposure on the growth and physiology (chlorophyll fluorescence, ion leakage, O2 evolution and pigmentation) of the red seaweed Gracilariopsis longissima was investigated. Of the various physiological end-points measured, growth proved to be the most sensitive with reductions in relative growth rate (RGR) observed at a concentration of 12.5 μg l-1 Cu, with zero growth above about 300 μg l-1. A significant increase in ion leakage and reduction in phycobiliprotein concentrations were evident, but only at the highest concentration tested (500 μg l-1), at which point shrinkage of apical tips also occurred. Photosynthetic rates, as measured by chlorophyll fluorescence and oxygen evolution, were first impaired at 250 μg l-1, with a 30% reduction in photosynthetic efficiency (Fv/Fm) and a 60% reduction in oxygen evolution. There were no discernible effects on respiration rates or chlorophyll a and β-carotene content over this concentration range. It was hypothesised that the observed uncoupling of growth and photosynthesis at low Cu concentrations might be explained by the release of dissolved organic matter (DOC), resulting in less available energy for growth. No such increase in DOC was apparent. Alternative explanations to account for the uncoupling, including the diversion of energy for maintenance of cell integrity and induction of protective mechanisms, are discussed. |
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