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Apoptotic cell death in rainbow trout (Oncorhynchus mykiss) and goldfish (Carassius auratus) ovarian follicles in vivo and in vitro
Wood, A.W.; Van Der Kraak, G.J. (2000). Apoptotic cell death in rainbow trout (Oncorhynchus mykiss) and goldfish (Carassius auratus) ovarian follicles in vivo and in vitro, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 295-297
In: Norberg, B. et al. (Ed.) (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales, more

Available in Authors 
    VLIZ: Proceedings [4254]

Keyword
    Marine

Authors  Top 
  • Wood, A.W.
  • Van Der Kraak, G.J.

Abstract
    We have initiated studies to examine the role of apoptosis in follicular atresia of teleost fish. Ovarian follicles from goldfish and rainbow trout were examined for evidence of oligonucleosomal fragmentation (apoptosis) before and after incubation (24-72 h) in serum-free medium. Electrophoretic size fractionation of 3'-end-labeled follicular DNA and in situ (TUNEL) analyses suggest that fish ovarian follicles are highly resistant to apoptosis, and that apoptotic cell death in vivo in healthy follicles is restricted to cells in the theca and surface epithelium. Notable species- and developmental stage-specific differences in susceptibility to apoptotic demise were also observed.

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