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Regulation of FSH (GTH I) and LH (GTH II) in coho salmon (Oncorhynchus kisutch): the action of recombinant human activin A and salmon testis extract
Davies, B.; Dickey, J.; Swanson, P. (2000). Regulation of FSH (GTH I) and LH (GTH II) in coho salmon (Oncorhynchus kisutch): the action of recombinant human activin A and salmon testis extract, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 486
In: Norberg, B. et al. (Ed.) (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales, more

Available in  Authors 
    VLIZ: Proceedings [4382]

Keyword
    Marine

Authors  Top 
  • Davies, B.
  • Dickey, J.
  • Swanson, P.

Abstract
    The action of recombinant human Activin A (rhActivin A) and a steroid/GnRH-free salmon testis extract on the production and release of gonadotropins in male coho at early stages of maturation has been investigated using a static dispersed pituitary cell culture system. rhActivin A produced a relatively weak increase (36%) in media FSH (LH remaining non-detectable) whilst, in contrast, the testis extract produced a large (157%) increase in FSH and LH rose to detectable levels. A multiplex RNase protection assay indicated that the testis extract and sGnRH, but not rhActivin A, significantly elevated alpha 1, alpha 2, FSH beta (LH beta non-detectable) subunit mRNA in pituitary cells incubated with these factors for 72 hours.

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