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Functional expression of recombinant seabream FSH and LH in baculovirus infected insect cells
Meiri, I.; Zmora, N.; Elizur, A. (2000). Functional expression of recombinant seabream FSH and LH in baculovirus infected insect cells, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 488
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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Keyword
    Marine/Coastal
Authors  Top 
  • Meiri, I.
  • Zmora, N.
  • Elizur, A.
Abstract
    The coding regions for the seabream (sb) alpha GtH and beta FSH or beta LH were engineered into the baculovirus (bv) expression vector pAcUW51, under the control of independent promoters. Expression resulted in subunit association and secretion of LH and FSH by the insect cells. A construct containing the full length beta LH cDNA, joined to the cDNA coding for the mature alpha subunit, was introduced into the pVL1393 vector to determine whether the active conformation of the heterodimer could be achieved when the two subunits were synthesized as a single polypeptide chain. Media containing the recombinant FSH and LH were applied in vitro to sb gonads, and secretion of estradiol 17 beta (E2), followed. Our results show that both conformations are capable of eliciting an in vitro biological response.
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