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A method for determining free fatty acids (FFA) in marine plankton and sediments by means of high performance liquid chromatographic analysis of their p-nitrobenzyl esters
Falkowski, L.; Pazdro, K.; Lewandowska, J.; Osterroht, C.; Slebioda, M. (1993). A method for determining free fatty acids (FFA) in marine plankton and sediments by means of high performance liquid chromatographic analysis of their p-nitrobenzyl esters. Oceanologia 34: 57-68
In: Oceanologia. Polish Academy of Sciences, Institute of Oceanology: Wroclaw; Gdansk. ISSN 0078-3234, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Falkowski, L.
  • Pazdro, K.
  • Lewandowska, J.
  • Osterroht, C.
  • Slebioda, M.

Abstract
    A method for determining free fatty acid content in marine plankton and sediments by HPCL analysis of their p-nitrobenzyl esters has been elaborated. The acids were extracted from lyophilised samples of plankton or sediment with a 2:1 chloroform-methanol mixture. They were isolated from the remaining lipids by extraction with alkaline water and reextraction, after acidification, with chloroform. The solvent was evaporated, the remaining substances dissolved in dichloromethane (DCM) and estrified with N,N'-dicyclohexyl-0-p-nitrobenzyl isourea (DcH-pNBiU). The reaction mixture was separated on a silicagel pad using a cyclohexaneethyl acetate mixture, the fractions containing the esters were evaporated, dissolved in an appropriate solvent and analysed by means of HPLC.

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