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A simple density separation technique for quantitative isolation of meiobenthos using the colloidal silica Ludox-TM
De Jonge, V.N.; Bouwman, L.A. (1977). A simple density separation technique for quantitative isolation of meiobenthos using the colloidal silica Ludox-TM. Mar. Biol. (Berl.) 42: 143-148
In: Marine Biology. Springer: Heidelberg; Berlin. ISSN 0025-3162, more
Peer reviewed article  

Also published as
  • De Jonge, V.N.; Bouwman, L.A. (1983). A simple density separation technique for quantitative isolation of meiobenthos using the colloidal silica Ludox-TM, in: Bouwman, L.A. Systematics, ecology and feeding biology of estuarine nematodes. pp. 9-14, more

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Authors  Top 
  • De Jonge, V.N., more
  • Bouwman, L.A.

Abstract
    A simple, quantitative density separation method is described. The method is based on differences in specific weight between meiobenthos and sediment. Nematodes and copepods could be separated from sediment and detritus when samples were suspended in Ludox-TM, a colloidal silica. Organisms float at the surface, while sediment particles sink. Results obtained with this new method were compared with the well-known decantation method. For a quantitative isolation of nematodes from sediments, rich in coarse detritus, a maximum volume of 7 cm SUP-3 sample could be used. For copepods this maximum was 13 cm SUP-3 . For such sediments the density method is more reliable than the decantation method. The time needed for sorting the meiobenthic organisms is reduced to about 30 per cent compared with the former method. The new method can be used for preserved as well as for fresh sediment samples and can also be applied for the isolation of small polychaetes, small oligochaetes, larvae of some macrofaunal groups and net-zooplankton.

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