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Genome size of Adriatic seagrasses
Koce, J.D.; Vilhar, B.; Bohanec, B.; Dermastia, M. (2003). Genome size of Adriatic seagrasses. Aquat. Bot. 77(1): 17-25. dx.doi.org/10.1016/s0304-3770(03)00072-x
In: Aquatic Botany. Elsevier Science: Tokyo; Oxford; New York; London; Amsterdam. ISSN 0304-3770, more
Peer reviewed article  

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Keywords
    DNA; Genomes; Sea grass; Alismatidae; Cymodocea nodosa (Ucria) Ascherson, 1870 [WoRMS]; Pisum sativum; Posidonia oceanica (Linnaeus) Delile, 1813 [WoRMS]; Zostera (Zostera) marina Linnaeus, 1753 [WoRMS]; Zostera (Zosterella) noltei Hornemann [WoRMS]; MED, Adriatic [Marine Regions]; Marine

Authors  Top 
  • Koce, J.D.
  • Vilhar, B.
  • Bohanec, B.
  • Dermastia, M.

Abstract
    Genome size (C-value) was measured in four species of Adriatic seagrasses with interphase-peak DNA image cytometry. The estimated 2C-value was 1.5 pg DNA for Zostera noltii (2n=12), 1.2 pg for Zostera marina (2n=12), 1.1 pg for Cymodocea nodosa (2n=28) and 6.2 pg for Posidonia oceanica, using Pisum sativum (2C-value=8.84 pg) as the calibration standard. Seagrass leaves were fixed in 4% buffered formaldehyde to mitigate stoichiometric error due to tannins and post-fixed in 3:1 methanol:acetic acid (MAA). DNA was stained with the Feulgen reaction after hydrolysis in 5 M HCl for 90 min at 20 °C. Comparison of genome size of seagrasses with the data for other species of Alismatidae indicated that the ancestral genome of Alismatidae was relatively small.

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