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Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia fransiscana
Nelis, H.J.; Merchie, G.; Lavens, P.; Sorgeloos, P.; De Leenheer, A.P. (1994). Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia fransiscana, in: (1994). IZWO Coll. Rep. 24(1994). IZWO Collected Reprints, 24: pp. chapter 1994
In: (1994). IZWO Coll. Rep. 24(1994). IZWO Collected Reprints, 24[s.n.][s.l.], more
In: IZWO Collected Reprints. Instituut voor Zeewetenschappelijk Onderzoek: Bredene & Oostende. ISSN 0772-1250, more

Also published as
  • Nelis, H.J.; Merchie, G.; Lavens, P.; Sorgeloos, P.; De Leenheer, A.P. (1994). Solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia fransiscana. Anal. Chem. 66(8): 1330-1333, more

Keywords
    Artemia franciscana Kellog, 1906 [WoRMS]; Marine; Brackish water

Authors  Top 
  • Nelis, H.J.
  • Merchie, G.
  • Lavens, P., more
  • Sorgeloos, P., more
  • De Leenheer, A.P.

Abstract
    Two producers are described for the solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia . The first one is based on reversed-phase ion pair extraction on an octadecylsilica cartridge using tetrabutylammonium as the counterion, followed by elution with methanol. In the second procedure ascorbic acid 2-sulfate is retained on a DEAE silica cartridge and eluted with 0.1M sodium salicylate. Both approaches were used as part of a quantitative reversed-phase ion pair liquid chromatographic method with detection at 254nm. The anion exchange extraction procedure is superior to its ion pair counterpart in terms of recovery (88.7±3,5% versus 63.0±12.7%), reproducibility (CVs of 3.6-7.7% versus 6.3-18.7%), and simplicity.

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