IMIS | Flanders Marine Institute
 

Flanders Marine Institute

Platform for marine research

IMIS

Publications | Institutes | Persons | Datasets | Projects | Maps
[ report an error in this record ]basket (0): add | show Printer-friendly version

Effect of different light regimes on the docosahexaenoic acid (DHA) content of Isochrysis aff. galbana (clone T-ISO)
Tzovenis, I.; De Pauw, N.; Sorgeloos, P. (1997). Effect of different light regimes on the docosahexaenoic acid (DHA) content of Isochrysis aff. galbana (clone T-ISO), in: IZWO Coll. Rep. 27(1997). IZWO Collected Reprints, 27: pp. chapter 28
In: (1997). IZWO Coll. Rep. 27(1997). IZWO Collected Reprints, 27[s.n.][s.l.], more
In: IZWO Collected Reprints. Instituut voor Zeewetenschappelijk Onderzoek: Bredene. ISSN 0772-1250, more

Also published as
  • Tzovenis, I.; De Pauw, N.; Sorgeloos, P. (1997). Effect of different light regimes on the docosahexaenoic acid (DHA) content of Isochrysis aff. galbana (clone T-ISO). Aquacult. Int. 5: 489-507, more

Available in  Authors 

Keywords
    Rhodomonas salina (Wislouch) D.R.A.Hill & R.Wetherbee, 1989 [WoRMS]; Marine

Authors  Top 
  • Tzovenis, I.
  • De Pauw, N., more
  • Sorgeloos, P., more

Abstract
    The culture of Isochrysis aff. galbana Green (T-ISO) in hatcheries is promoted for its high docosahexaenoic acid (DHA) content which favours the successful rearing of aquatic larvae. In this paper we identified the need for well-defined culture conditions of microalgae if sufficient DHA content is the final goal. Using photoperiods of 24:0h, 12:12h and 16:8h L/D combined with photon flux densities (PFD's) of 110, 220 and 460 µE m-2 s-1, batch cultures of T-ISO were sampled for DHA content in the exponential as well as the post-exponential (light-limited) growth phase. The various light regimes resulted in differences of DHA content of T-ISO revealing influences and strong interactions among the three variables studied. For fast and economic production of T-ISO cells rich in DHA it is recommended to harvest batch cultures at the end of the exponential phase using high photon flux densities (PFD's) under 12:12 h L/D cycles. The same regime or lower PFD under 24:0 h L/D seem to be sufficient for a continuous culture optimization scheme. In any case a strict following of culture protocols is very important since there is evidence for coexistence of different clones within the parental cultures leading to different results depending on which one prevails.

All data in IMIS is subject to the VLIZ privacy policy Top | Authors