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Research on the development of a standardized ecotoxicological test on marine nematodes: 1. Culturing conditions and criteria for two monhysterids, Monhystera disjuncta and Monhystera microphthalma
Vranken, G.; Van Brussel, D.; Vanderhaeghen, R.; Heip, C.H.R. (1985). Research on the development of a standardized ecotoxicological test on marine nematodes: 1. Culturing conditions and criteria for two monhysterids, Monhystera disjuncta and Monhystera microphthalma, in: (1985). IZWO Coll. Rep. 15(1985). IZWO Collected Reprints, 15: pp. chapter 17
In: (1985). IZWO Coll. Rep. 15(1985). IZWO Collected Reprints, 15[s.n.][s.l.], more
In: IZWO Collected Reprints. Instituut voor Zeewetenschappelijk Onderzoek: Bredene & Oostende. ISSN 0772-1250, more

Also published as
  • Vranken, G.; Van Brussel, D.; Vanderhaeghen, R.; Heip, C.H.R. (1984). Research on the development of a standardized ecotoxicological test on marine nematodes: 1. Culturing conditions and criteria for two monhysterids, Monhystera disjuncta and Monhystera microphthalma, in: Persoone, G. et al. (Ed.) (1984). Proceedings of the International Symposium on Ecotoxicological Testing for the Marine Environment, Ghent, Belgium, september 12-14, 1983: volume 2. pp. 159-183, more
  • Vranken, G.; Van Brussel, D.; Vanderhaeghen, R.; Heip, C.H.R. (1984). Research on the development of a standardized ecotoxicological test on marine nematodes: 1. Culturing conditions and criteria for two monhysterids, Monhystera disjuncta and Monhystera microphthalma, in: Coomans, A. et al. (Ed.) (1984). Geconcerteerde Onderzoeksacties Oceanografie: Progress Report 1983. Ecological and ecotoxicological studies of benthos of the Southern Bight of the North Sea. pp. 167-185, more

Keywords
    Toxicity tests; Monhystera disjuncta (Bastian, 1865) [WoRMS]; Monhystera microphthalma de Man, 1880 [WoRMS]; Marine; Brackish water

Authors  Top 
  • Vranken, G.
  • Van Brussel, D.
  • Vanderhaeghen, R.
  • Heip, C.H.R., more

Abstract
    The brackish-water nematodes Monhystera disjuncta and Monhystera microphtalma were chosen as test organisms for ecotoxicological studies. Both species are locally abundant, have short life cycles and can be maintained for several generations under laboratory conditions. They reproduce very well on a sloppy bacto-agar (0.5-0.8%) made up with water of the natural habitat, enriched with 1% Vlasblom medium (Vlasblom, pers. commun.) and 1% silicium (15g.l-1), reaching densities of approximately 1000 individuals.cm-2. Starting from such agnotobiotic cultures, a chemically defined artificial medium has been developed. This medium consists of 0.5% bacto-agar in artificial seawater, enriched with 1% silicium (15 g.l-1, 1% amino acids (modified after Lee et al, 1970) and 1% modified Provasoli-Walne nutrient medium (ratio 1:5) (Ukeles, 1976; Vlasblom, pers. commun.), with a mixture of unidentified bacteria as food supply. A series of experiments was conducted in order to cultivate these nematodes under gnotobiotic circumstances with monospecific bacterial cultures. Several marine bacterial strains were isolated and tested . Two of them were suitable if a mixture of sterols (1%) was added to the medium.

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