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Protein digestion in juvenile turbot (Scophthalmus maximus) and effects of dietary administration of Vibrio proteolyticus
De Schrijver, R.; Ollevier, F.P. (2000). Protein digestion in juvenile turbot (Scophthalmus maximus) and effects of dietary administration of Vibrio proteolyticus, in: (2000). VLIZ Coll. Rep. 30(2000). VLIZ Collected Reprints: Marine and Coastal Research in Flanders, 30: pp. chapter15
In: (2000). VLIZ Coll. Rep. 30(2000). VLIZ Collected Reprints: Marine and Coastal Research in Flanders, 30. Flanders Marine Institute (VLIZ): Oostende, more
In: VLIZ Collected Reprints: Marine and Coastal Research in Flanders. Vlaams Instituut voor de Zee: Oostende. ISSN 1376-3822, more

Also published as
  • De Schrijver, R.; Ollevier, F.P. (2000). Protein digestion in juvenile turbot (Scophthalmus maximus) and effects of dietary administration of Vibrio proteolyticus. Aquaculture 186(1-2): 107-116. dx.doi.org/10.1016/S0044-8486(99)00372-5, more

Keywords
    Aquaculture; Diets; Digestibility; Digestion; Feeding experiments; Fish culture; Food conversion; Nitrogen; Probiotics; Probiotics; Proteins; Scophthalmus maximus; Stomach; Scophthalmus maximus (Linnaeus, 1758) [WoRMS]; Vibrio proteolyticus Baumann, Baumann & Mandel, 1971 [WoRMS]; Marine
Author keywords
    turbot; Scophthalmus maximus; protein digestion; Vibrio proteolyticus; probiotics

Authors  Top 
  • De Schrijver, R., more
  • Ollevier, F.P., more

Abstract
    The trial was made to study the protein digestion in juvenile turbot during passage of digesta from stomach to rectum. Moreover, diet supplementation with the potential probiotic bacteria, Vibrio proteolyticus, was evaluated with regard to protein digestion. For a 3-week period, fish (25-30 g) were fed by oral intubation and received either a liquid mixture consisting of 40% nonpurified control diet and 60% water or this mixture supplemented with 10 10 viable V. proteolyticus ml -1. Daily dry matter was 1.5% body weight. As digesta progressed from the stomach to the foregut, hindgut and rectum, the increase in apparent nitrogen digestibility was accompanied by higher ammonia contents, suggesting substantial involvement of the microbiota in protein degradation in the distal segments of the gastrointestinal tract. Water-soluble nitrogen contents were significantly higher in the foregut, presumably corresponding with considerable protein digestion by secreted endogenous enzymes in this digestive segement. Over 65% of the soluble protein in all four parts of the tract had MW < 10,000. The amount of soluble protein and peptides with MW < 1000 decreased significantly during transit. This was also found for the proportion of the 10,000-20,000 MW proteins, whereas the highest MW category (>200,000) increased. Ingestion of V. proteolyticus tended to stimulate apparent nitrogen digestibility (P<0.1). This effect corresponded with increased protein degradation in the proximal intestine as was shown by the significantly elevated fraction of soluble proteins with MW<1000.

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