|The mucous secretory apparatus of the free urn cell ofSipunculus nudus|
Bang, B.G.; Bang, F.B. (1976). The mucous secretory apparatus of the free urn cell ofSipunculus nudus. Cah. Biol. Mar. 17(4): 423-432
In: Cahiers de Biologie Marine. Station Biologique de Roscoff: Paris. ISSN 0007-9723, more
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The secretory apparatus of 2 distinct secretory systems in the same cell has been identified in living mucociliated cells. Each type of secretion falls to stain with the supravital dye which stains the other. One secretion is slowly produced, is membrane bound, and stains metachromatically with basic aniline dyes in thiazin, oxazin and azin groups. The other secretion is a free-flowing mucus which is induced by specific stimuli; the violet form of the basic azo dye Janus green is concentrated at the site of synthesis of this system, and the dye is then bound to the emerging mucus is either its violet or its bluegreen form, depending on the stimulus. The test cells are 'free urn cells', a population of mucociliated epithelial cells in the coelomic fluid of S. nudus. They are cultured and tested in their own serum. Stumuli which consistently induce secretion of different morphological types of mucus by urn cells include 7 human body fluids. Janus green is also concentrated and secreted by mucous gland cells in organ cultures of chick nasal turbinates.