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Effect of three xenobiotic compounds on glutathione S-transferase in the clam Ruditapes decussatus
Hoarau, P.; Garello, G.; Gnassia-Barelli, M.; Roméo, M.; Girard, J.-P. (2004). Effect of three xenobiotic compounds on glutathione S-transferase in the clam Ruditapes decussatus. Aquat. Toxicol. 68(1): 87-94. dx.doi.org/10.1016/j.aquatox.2004.03.001
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X, more
Peer reviewed article  

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Keywords
    Biomarkers; Methoxychlor; Transferases; Ruditapes decussatus (Linnaeus, 1758) [WoRMS]; MED, France, Languedoc-Roussillon, Thau Lagoon [Marine Regions]; Marine

Authors  Top 
  • Hoarau, P.
  • Garello, G.
  • Gnassia-Barelli, M.
  • Roméo, M., correspondent
  • Girard, J.-P.

Abstract
    The effects of 4,4′DDE, methoxychlor and imidazole were studied on glutathione S-transferase activities in the gills and hepatopancreas of the clam Ruditapes decussatus. The contamination doses were 0.14 µM for 4,4′DDE, 0.14 µM for methoxychlor and 25 M for imidazole. GST activities were spectrophotometrically measured. SDS-PAGE and isoelectric focusing (IEF) were used to separate the different GST isoforms in control and treated animals, followed by Western blotting performed with anti-alpha, anti-mu and anti-pi GST anti-sera. In the hepatopancreas, GST-CDNB activities were always two to five-fold lower than in the gills where the activities were significantly increased after exposure to 4,4′DDE (ca. 1.6-fold) and to methoxychlor (ca. 1.3-fold) compared to the controls (ca. 3 µmol min−1 mg−1protein) whereas they remained unchanged after treatment with imidazole. When using glutathione S-transferase anti-alpha, anti-mu and anti-pi anti-sera, a single 26 kDa polypeptide was observed in the hepatopancreas and in the gills in all the tested conditions. Five GST subunits were observed after IEF showing greater immuno-reactivity with the anti-pi mammalian class antiserum than with the anti-alpha or anti-mu mammalian anti-sera. One isoform of pI 5.77 was particularly induced by 4,4′DDE and methoxychlor; it was recognized by the three anti-sera tested and seemed to be more efficient in the gills than in the hepatopancreas. This isoform may play a role in organochlorine detoxication.

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