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DNA primers for amplification of mitochondrial cytochrome c oxidase Subunit 1 from diverse metazoan invertebrates
Folmer, O.; Black, M.; Hoeh, W.; Lutz, R.; Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase Subunit 1 from diverse metazoan invertebrates. Mol. Mar. Biol. Biotech. 3(5): 294-299
In: Molecular Marine Biology and Biotechnology. Springer-Verlag: New York. ISSN 1053-6426, more
Peer reviewed article  

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Keywords
    Cytochromes; DNA; Marine invertebrates; Mitochondria; Polymerase chain reaction; Annelida [WoRMS]; Arthropoda [WoRMS]; Coelenterata [WoRMS]; Echinodermata [WoRMS]; Echiura [WoRMS]; Mollusca [WoRMS]; Vestimentifera [WoRMS]; Marine

Authors  Top 
  • Folmer, O.
  • Black, M.
  • Hoeh, W.
  • Lutz, R.
  • Vrijenhoek, R.

Abstract
    We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at the species and higher taxonomic levels.

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