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CASA: a quantitative and rapid method for the assessment of sperm quality in fish
Rurangwa, E.; Volckaert, F.A.M.J.; Huyskens, G.; Kime, D.E.; Ollevier, F.P. (2001). CASA: a quantitative and rapid method for the assessment of sperm quality in fish, in: Hendry, C.I. et al. (Ed.) Larvi 2001: 3rd fish and shellfish larviculture symposium Gent, Belgium, September 3-6, 2001. Special Publication European Aquaculture Society, 30: pp. 529
In: Hendry, C.I. et al. (Ed.) (2001). Larvi 2001: 3rd fish and shellfish larviculture symposium Gent, Belgium, September 3-6, 2001. Special Publication European Aquaculture Society, 30. European Aquaculture Society: Oostende. XX1, 663 pp., more
In: Special Publication European Aquaculture Society. European Aquaculture Society: Bredene. ISSN 0774-0689, more

Available in  Authors 
    VLIZ: Proceedings 20/3 [60621]
Document type: Conference paper

Keywords
    Marine; Brackish water; Fresh water

Authors  Top 
  • Rurangwa, E., more
  • Volckaert, F.A.M.J., more
  • Huyskens, G.
  • Kime, D.E.
  • Ollevier, F.P., more

Abstract
    In the past decade, attention of the fish farming industry has been directed much more towards the quality of eggs and larvae than to that of sperm, even though rearing conditions may affect the sperm quality of male broodstock. Motility is most commonly used to evaluate the quality of sperm, since they must be motile to achieve fertilization. However, the assessment of sperm quality in fish has for long relied on subjective estimates of motility characteristics, the value of which were and are still questionable in predicting fertility. Computer Assisted Sperm Analysis (CASA) systems were initially developed to examine sperm quality in mammals and birds and have only recently been applied to fish sperm. CASA can play an important role in aquaculture as it can rapidly and quantitatively examine the effects of water chemistry and food quality, temperature manipulation, photoperiod, or holding conditions on sperm quality and hence, fertilizing ability of farmed fish. CASA has, for example, been used to select the most appropriate extenders and cryoprotectants for freezing fish sperm and to assess the viability of spermatozoa after storage in liquid nitrogen. The use of computer-calculated motility as a measure of sperm quality in fish is reviewed.

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