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Isolation and characterization of heterotrophic aerobic thermophilic bacteria from three geographically separated deep-sea hydrothermal vents
Birrien, J.L.; Marteinsson, V.T.; Jeanthon, C.; Prieur, D. (1997). Isolation and characterization of heterotrophic aerobic thermophilic bacteria from three geographically separated deep-sea hydrothermal vents, in: Biologie des sources hydrothermales profondes = Biology of deep-sea hydrothermal vents: Journées d'échanges du Programme DORSALES = DORSALES Workshop Roscoff 6-8 octobre 1997. Cahiers de Biologie Marine, 38(2): pp. 114
In: (1997). Biologie des sources hydrothermales profondes = Biology of deep-sea hydrothermal vents: Journées d'échanges du Programme DORSALES = DORSALES Workshop Roscoff 6-8 octobre 1997. Cahiers de Biologie Marine, 38(2)[s.n.][s.l.]. 111-149 pp., more
In: Cahiers de Biologie Marine. Station Biologique de Roscoff: Paris. ISSN 0007-9723, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Birrien, J.L.
  • Marteinsson, V.T.
  • Jeanthon, C.
  • Prieur, D.

Abstract
    A total of 126 heterotrophic aerobic thermophilic bacteria (80 sporeforming and 46 non-sporulating rods) were isolated from samples collected at three geographically separated deep-sea hydrothermal vents: Snakepit (Mid-Atlantic Ridge) at 3600 m depth, Guaymas Basin (Gulf of California) and Lau Basin (SW Pacific ocean) both at 2000 m depth. Numerical classification based on the phenotypic features of the isolates was performed with unweighed average linkage (UPGMA) cluster analysis of a similarity matrix derived from the simple matching (S sub(SM)) coefficient. Non-sporulating isolates consisted of pleomorphic rods, single cells or pairs and formed filaments of variable length. They grew at 70 degree C or some up to 80 degree C, were halotolerant and unable to grow anaerobically. Five main clusters were formed which were defined for at least 80% similarity. The reference strain Thermus scotoductus (X-1) clustered with one isolate (GY121 1) at a level of 83% while other Thermus reference strains showed less than 55% similarity with the deep-sea isolates. Hybridization results between the cluster consisting of isolate (GY 121 1 ) and 12 other deep-sea strains isolated later from the same sample, showed 69 to 76% homology with Thermus thermophilus (HB8). All deep-sea sporeforming isolates were halotolerant and grew aerobically above 65 degree C. Nine main phena defined for at least 83% similarity were formed. Seven phena were composed exclusively of strains isolated from the same site (4 from Mid-Atlantic Ridge, I from Guaymas Basin and 2 from Lau Basin). The majority of the Lau Basin isolates clustered with six reference strains in one phenon, whereas isolates from Mid-Atlantic Ridge and Guaymas Basin were found separated from this phenon at 69% similarity level. The other five high temperature Bacillus reference strains showed less than 69% similarity with the deep-sea isolates.

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