| one publication added to basket [66265] | Quantitative and qualitative analysis of two series of chitin secreting glands sections in Riftia pachyptila
Boudier, Th.; Zbinden, M.; Gaill, F. (1997). Quantitative and qualitative analysis of two series of chitin secreting glands sections in Riftia pachyptila, in: Biologie des sources hydrothermales profondes = Biology of deep-sea hydrothermal vents: Journées d'échanges du Programme DORSALES = DORSALES Workshop Roscoff 6-8 octobre 1997. Cahiers de Biologie Marine, 38(2): pp. 115
In: (1997). Biologie des sources hydrothermales profondes = Biology of deep-sea hydrothermal vents: Journées d'échanges du Programme DORSALES = DORSALES Workshop Roscoff 6-8 octobre 1997. Cahiers de Biologie Marine, 38(2)[s.n.][s.l.]. 111-149 pp., more
In: Cahiers de Biologie Marine. Station Biologique de Roscoff: Paris. ISSN 0007-9723; e-ISSN 2262-3094, more
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| Authors | | Top |
- Boudier, Th.
- Zbinden, M.
- Gaill, F.
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| Abstract |
Riftia pachyptila is known to be a model for the chitin secretion study. Its secretory system is amplified and the chitin production rate is high. The chitin secreting gland consists in a main lumen surrounded by a one cell layer epithelium. The cells of this epithelium may present a sublumen that is connected or not to the gland main lumen. Chitin crystallises are synthesized in differentiated structures, the cups, located in the sublumen surface. Boudier et al (1997) proposed a classification of the cells involved in the secretion. The chitin secreting cells classification is based on the connection and the shape of their sublumen. Semi-thin cross and longitudinal sections of Riftia pachyptila chitin secreting glands, from specimens collected during the HOT 96 Scientific Cruise, were examinated. Gland cells were numbered, their typology and their location on the gland noticed. Observations on the cytological aspects of these cells, like the nucleus size, the cups location, were performed. Numerical images of these sections were obtained via a video system mounted on a microscope. The calculation of the chitin potential stock was done on the whole gland. Each structures - epithelium, main lumen and sublumina - areas were obtained, for each sections, from the numerical images thanks to an image analysis software. |
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