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Detection of the whole-cell hybridization and isolation of new thermophilic microorganisms from deep-sea hydrothermal vents
Jeanthon, C.; Harmsen, H.J.M.; L'Haridon, S.; Cilia, V.; Gambacorta, A.; Prieur, D. (1997). Detection of the whole-cell hybridization and isolation of new thermophilic microorganisms from deep-sea hydrothermal vents, in: Biologie des sources hydrothermales profondes = Biology of deep-sea hydrothermal vents: Journées d'échanges du Programme DORSALES = DORSALES Workshop Roscoff 6-8 octobre 1997. Cahiers de Biologie Marine, 38(2): pp. 126
In: (1997). Biologie des sources hydrothermales profondes = Biology of deep-sea hydrothermal vents: Journées d'échanges du Programme DORSALES = DORSALES Workshop Roscoff 6-8 octobre 1997. Cahiers de Biologie Marine, 38(2)[s.n.][s.l.]. 111-149 pp., more
In: Cahiers de Biologie Marine. Station Biologique de Roscoff: Paris. ISSN 0007-9723, more
Peer reviewed article  

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Keyword
    Marine

Authors  Top 
  • Jeanthon, C.
  • Harmsen, H.J.M.
  • L'Haridon, S.
  • Cilia, V.
  • Gambacorta, A.
  • Prieur, D.

Abstract
    The diversity of the thermophilic microbial community thriving in deep-sea hydrothermal chimneys was assessed by the combined use of traditional culture-based enrichments of strictly anacrobic thermophiles and whole-cell hybridization with domain-, kingdom-, and group-specific fluorescent-oligonucleotide probes based on 16S rRNA. By both methods, the largest amounts of microorganisms were found in the upper and outer parts of the chimneys, although even the inner parts contained culturable and detectable amounts of cells. Different morphotypes of thermophilic and hyperthermophilic microorganisms were enriched and detected in samples. Our data clearly indicate that the morphological diversity observed by using whole-cell hybridization is much larger than that assessed by use of culture-based enrichments. We developed four 16S rRNA targeted oligonucleotide probes encompassing most of the thermophilic members of the genus Bacillus, most species of the genus Thermus, the genera Thermotoga and Thermosipho and the Aquificales order to identify and quantify cells from some of the previously analysed chimney samples.

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