Organization of the mitochondrial genome of Antarctic krill Euphausia superba (Crustacea: Malacostraca)
Machida, R.J.; Miya, M.U.; Yamauchi, M.M.; Nishida, M.; Nishida, S. (2004). Organization of the mitochondrial genome of Antarctic krill Euphausia superba (Crustacea: Malacostraca). Mar. Biotechnol. 6(3): 238-250. https://dx.doi.org/10.1007/s10126-003-0016-6
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236, more
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| Keywords |
Acids > Organic compounds > Organic acids > Nucleic acids > DNA
Biological phenomena > Evolution
Biology > Genetics
Cell constituents > Cell organelles > Mitochondria
Fauna > Aquatic organisms > Aquatic animals > Shellfish > Marine organisms > Marine crustaceans
Materials > Coating materials > Primers
Nucleic compounds > Nucleic acids > Dna > Mitochondrial dna
Crustacea [WoRMS]; Euphausia superba Dana, 1850 [WoRMS]; Malacostraca [WoRMS]
Marine/Coastal |
| Authors | | Top |
- Machida, R.J.
- Miya, M.U.
- Yamauchi, M.M.
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| Abstract |
We determined the nearly complete DNA sequence of the mitochondrial genome of Antarctic krill Euphausia superba (Crustacea: Malacostraca), one of the most ecologically and commercially important zooplankters in Antarctic waters. All of the genome sequences were purified by gene amplification using long polymerase chain reaction (PCR), and the products were subsequently used as templates for either direct sequencing using a primer-walking strategy or nested PCR with crustacea-versatile primers. Although we were unable to determine a portion of the genome owing to technical difficulties, the sequenced position, 14,606 bp long, contained all of the 13 protein-coding genes, 19 of the 22 transfer RNA genes, and the large subunit as well as a portion of the small subunit ribosomal RNA genes. Gene rearrangement was observed for 3 transfer RNA genes (tRNACys, tRNATyr, and tRNATrp) and the 2 leucine tRNA genes. |
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