| one publication added to basket [68503] | Characterization of proteases in the digestive system of spiny lobster (Panulirus interruptus)
Celis-Guerrero, L.E.; García-Carreño, F.L.; Navarrete del Toro, M.A. (2004). Characterization of proteases in the digestive system of spiny lobster (Panulirus interruptus). Mar. Biotechnol. 6(3): 262-269. http://dx.doi.org/10.1007/s10126-003-0032-6
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236, more
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| Keywords |
Anatomical structures > Digestive system
Chymotrypsin
Enzymes > Hydrolases > Peptide hydrolases > Proteinases > Serine proteinases > Chymotrypsin
Enzymes > Hydrolases > Peptide hydrolases > Proteinases > Serine proteinases > Trypsin
Proteases
Spiny lobsters
Trypsin
Panulirus interruptus (Randall, 1840) [WoRMS]
Marine/Coastal |
| Author keywords |
spiny lobster; Panulirus interruptus; proteases; digestive system;trypsin; chymotrypsin |
| Authors | | Top |
- Celis-Guerrero, L.E.
- García-Carreño, F.L.
- Navarrete del Toro, M.A.
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| Abstract |
Enzymes responsible for digestion of food protein were evaluated and characterized in red lobster (Panulirus interruptus). Several tissues, organs, and body fluids were analyzed. The same composition of proteases was found in gastric juice, midgut gland, and intestinal contents. Using specific substrates and inhibitors, we indentified several isotrypsins and isochymotrypsins by gel electrophoresis. Protease activity was found at pH 3 and reduced by using pepstatin A. Operational variables of enzymes were characterized for management of future studies and potential biotechnologies. Types and activities of lobster digestive enzymes constitute background information to study the digestive abilities of the organism further, and will lead to understanding nutritional needs and feeding ecology, mainly because decapods display unique morphologic, metabolic, and behavioral changes during their life cycle. Also, such enzymes become alternative tools for use in biotechnologies. |
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