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The effect of para-nonylphenol on Japanese eel (Anguilla japonica) spermatogenesis in vitro
Miura, C.; Takahashi, N.; Michino, F.; Miura, T. (2005). The effect of para-nonylphenol on Japanese eel (Anguilla japonica) spermatogenesis in vitro. Aquat. Toxicol. 71(2): 133-141.
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X, more
Peer reviewed article  

Available in  Authors 

    Endocrinology; Phenols; Sertoli cells; Spermatogenesis; Anguilla japonica Temminck & Schlegel, 1846 [WoRMS]; Marine
Author keywords
    nonylphenol; Japanese eel; spermatogenesis; endocrine disruption;11-ketotestosterone; testis; sertoli cells; estradiol-17 beta

Authors  Top 
  • Miura, C.
  • Takahashi, N.
  • Michino, F.
  • Miura, T.

    Endocrine disrupters have been recognized to interfere with endocrine systems that regulate reproduction, for example, by mimicking or inhibiting the action of endogenous sex steroid hormones including estradiol-17β (E2). In the present study, we examined the effect of an endocrine disrupter, para-nonylphenol (p-NP) on spermatogenesis, and compared it with the action of E2, using an eel testicular organ culture system. p-NP alone stimulated early spermatogonial renewal in the same manner as E2. Neither induced further progress in spermatogenesis. In the presence of 11-ketotestosterone (11-KT), the major androgen in teleosts, p-NP did not prevent the 11-KT-induced progress in spermatogenesis. However, this treatment enlarged the Sertoli cells. Electron microscopic observation revealed that enlarged Sertoli cells contained well-developed organelles. Moreover, the proportion of germ cells appeared to have decreased as a result of Sertoli cell hypertrophy. These results clearly show that p-NP has an effect on Sertoli cells in the presence of an androgen (11-KT), potentially disturbing 11-KT-induced spermatogenesis.

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