|Chlorophyll fluorescence measurements as a means for estimation of primary production|
Slooten, L. (1996). Chlorophyll fluorescence measurements as a means for estimation of primary production, in: Baeyens, J. et al. (Ed.) Integrated Marine System Analysis. European Network for Integrated Marine System Analysis. FWO Vlaanderen: minutes of the first network meeting (Brugge, 29.02.96-02.03.96). pp. 68-102
In: Baeyens, J.; Dehairs, F.A.; Goeyens, L. (Ed.) (1996). Integrated Marine System Analysis. European Network for Integrated Marine System Analysis. FWO Vlaanderen: minutes of the first network meeting (Brugge, 29.02.96-02.03.96). Vrije Universiteit Brussel. Laboratorium voor Analytische Chemie: Brussel. 217 pp., more
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VLIZ: Aquatic Ecology 10/2 
|Document type: Conference paper|
This review provides an introduction to the use of chlorophyll fluorescence measurements for the estimation of primary production. The photosynthetic electron transport chain is briefly described, with emphasis on reactions taking place in the photosystem II reaction center, inasmuch as they have a role in the modulation of the chlorophyll fluorescence yield. The equations governing the relationship between the chlorophyll fluorescence yield and the quantum yield for electron transport through photosystem II are derived. At least under well-defined conditions this quantum yield is proportional to the quantum yield for CO2 fixation. This is the basis for a rapid and nonintrusive method to obtain rates of CO2-assimilation, provided that allowance is made for a number of complications. These complications are also described, and include: uncertainty in the estimation of the amount of light absorbed by photosystem II; photoinhibition, which may affect the relationship between the chlorophyll fluorescence yield, and the quantum yield for electron transfer through photosystem II; and the fact that not all of the electrons transported through photosystem II end up in assimilated carbon.