|Pulse-amplitude-modulation-fluorescence (PAM) a tool for fast assessment of primary productivity in the sea?|
Hartig, P.; Colijn, F. (1996). Pulse-amplitude-modulation-fluorescence (PAM) a tool for fast assessment of primary productivity in the sea?, in: Baeyens, J. et al. (Ed.) Integrated Marine System Analysis. European Network for Integrated Marine System Analysis. FWO Vlaanderen: minutes of the first network meeting (Brugge, 29.02.96-02.03.96). pp. 103-121
In: Baeyens, J.; Dehairs, F.A.; Goeyens, L. (Ed.) (1996). Integrated Marine System Analysis. European Network for Integrated Marine System Analysis. FWO Vlaanderen: minutes of the first network meeting (Brugge, 29.02.96-02.03.96). Vrije Universiteit Brussel. Laboratorium voor Analytische Chemie: Brussel. 217 pp., more
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VLIZ: Aquatic Ecology 10/2 
|Document type: Conference paper|
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- Hartig, P.
- Colijn, F., more
Analysis of the kinetics of chlorophyll fluorescence quenching can give qualitative information on the functioning and the organisation of the photosynthetic apparatus. For higher plants a linear relation between fluorescence yield and electron transport has been observed. Edwards and Baker (1993) concluded that under a wide range of conditions the fluorescence yield can be used to predict accurately and rapidly CO2 assimilation rates in maize. Up to now it is an open question whether it is also possible to calculate the production rates of phytoplankton by analysis and measurement of fluorescence yield. This would give us a new tool for fast measurements of primary productivity in the sea. In order to assess the usefulness of the (Pulse-Amplitude-Modulated) PAM-fluorescence method to estimate primary production in marine phytoplankton we have examined the relation between the rate of relative photosystem II electron transport determined with the PAM-fluorescence technique and the rate of carbon fixation as measured with the conventional 14C-technique for different marine phytoplankton species (e.g., Skeletonema costatum, Thalassiosira weissflogii, Prorocentrum redfieldii, Dunaniella spec.) and microphytobenthos communities under different irradiance levels. A short overview about the PAM-fluorescence technique is presented to understand the main principles of this new technique.