|Testosterone metabolism modulation in Neomysis integer: a biomarker for endocrine disruption|
|Poelmans, S.; Verslycke, T.; Noppe, H.; Van Hoof, N.; De Brabander, H.; Janssen, C.R. (2005). Testosterone metabolism modulation in Neomysis integer: a biomarker for endocrine disruption, in: Mees, J. et al. (Ed.) (2005). VLIZ Young Scientists' Day, Brugge, Belgium 25 February 2005: book of abstracts. VLIZ Special Publication, 20: pp. 52|
|In: Mees, J.; Seys, J. (Ed.) (2005). VLIZ Young Scientists' Day, Brugge, Belgium 25 February 2005: book of abstracts. VLIZ Special Publication, 20. Vlaams Instituut voor de Zee (VLIZ): Oostende. X, 129 pp., more|
|In: VLIZ Special Publication. Vlaams Instituut voor de Zee (VLIZ): Oostende. ISSN 1377-0950, more|
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|Document types: Conference paper; Summary|
Neomysis integer (Leach, 1814) [WoRMS]; Marine
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Cytochrome P450-dependent monooxygenases (P450s) are important enzymes in the metabolic system and are involved in the regulation of hormone synthesis and in the detoxification and/or activation of xenobiotics. P450s are found in virtually all aerobic organisms, from invertebrates to vertebrates. A number of endocrine disruptors are suspected of exerting their effects through disruption of normal CYP function.
Consequently, alterations of steroid hormone metabolism by CYP modulators can be an important tool to study potential effects of endocrine disruptors in invertebrates. The invertebrate mysid Neomysis integer (Crustacea; Mysidacea) was used to asses changes in the testosterone metabolism after a short-term exposure to four environmentally relevant CYP modulators. N. integer was exposed to sublethal concentrations of different endocrine disruptors, tributyltin (TBT), nonylphenol (NP) and phenobarbital (PB), and subsequently to testosterone. Identification and quantification of the steroid components was performed using liquid chromatography coupled with multiple mass spectrometry.
The effects of the different CYP modulators on phase I and phase II testosterone metabolism are discussed and compared. We conclude that the testosterone metabolism of N. integer is a sensitive endpoint to detect endocrine disruption of chemicals after a short- term exposure.