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Measurement of EROD activity: caution on spectral properties of standards used
Radenac, G.; Coteur, G.; Danis, B.; Dubois, P.; Warnau, M. (2004). Measurement of EROD activity: caution on spectral properties of standards used. Mar. Biotechnol. 6(4): 307-311. dx.doi.org/10.1007/s10126-004-3014-4
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228, more
Peer reviewed article  

Available in Authors 
    VLIZ: Open Repository 280392 [ OMA ]

Keywords
    Biomarkers; Fluorimeters; Spectral analysis; Marine
Author keywords
    EROD; ethoxyresorufin; resorufin; spectral characteristics; fluorimetry; biomarker

Authors  Top 
  • Radenac, G.
  • Coteur, G.
  • Danis, B., more
  • Dubois, P., more
  • Warnau, M.

Abstract
    The activity of the enzyme 7-ethoxy-resorufin-O-deethylase (EROD) has been extensively used in biomonitoring studies for more than a decade. Although the analytical procedure is simple, it is often poorly characterized. In this study spectral properties of particular standard compounds used to measure EROD activity (ethoxyresorufin and resorufin, standards from Molecular Probes) were tested in order to optimize excitation and emission wavelengths to be used in the fluorimetric assay of EROD activity. The optimal excitation wavelength for the detection of resorufin was 560 nm. At this wavelength the excitation represents only 37% of its maximum level for ethoxyresorufin, while it represents 86% for resorufin. This allows discrimination between the fluorescence emitted by both standards, favoring the formed resorufin. Our results demonstrate that any analytical work using spectrofluorometry to measure EROD activity should be preceded by precise determination of the spectral characteristics of each set of standards used.

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