A highly active alkaline phosphatase from the marine bacterium Cobetia
Plisova, E.Y.; Balabanova, L.A.; Ivanova, E.P.; Kozhemyako, V.B.; Mikhailov, V.V.; Agafonova, E.V.; Rasskazov, V.A. (2005). A highly active alkaline phosphatase from the marine bacterium Cobetia. Mar. Biotechnol. 7(3): 173-178. https://dx.doi.org/10.1007/s10126-004-3022-4
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228; e-ISSN 1436-2236, more
|   |
| Keywords |
Alkaline phosphatase
Chemical compounds > Alkaline earth metal compounds
Microorganisms > Bacteria
Processing > Purification
Properties
Proteins > Enzymes > Hydrolases > Esterases > Phosphoric monoester hydrolases > Alkaline phosphatase
Purification
Purification
Specificity
Cobetia marina Cobet, A.B., Wirsen, C. & Jones, 1970 [WoRMS]
Marine/Coastal |
| Authors | | Top |
- Plisova, E.Y.
- Balabanova, L.A.
- Ivanova, E.P.
- Kozhemyako, V.B.
|
- Mikhailov, V.V.
- Agafonova, E.V.
- Rasskazov, V.A.
|
|
| Abstract |
An alkaline phosphatase with unusually high specific activity has been found to be produced by the marine bacterium Cobetia marina (strain KMM MC-296) isolated from coelomic liquid of the mussel Crenomytilus grayanus. The properties of enzyme, such as a very high specific activity (15000 DE U/1 mg of protein), no activation with divalent cations, resistance to high concentrations of inorganic phosphorus, as well as substrate specificity toward 5′ nucleotides suggest that the enzyme falls in an intermediate position between unspecific alkaline phosphatases (EC 3.1.3.1) and 5′ nucleotidases (EC 3.1.3.5). |
|
