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Isolation and characterization of twenty microsatellite loci in Japanese sea cucumber (Stichopus japonicus)
Kanno, M.; Li, Q.; Kijima, A. (2005). Isolation and characterization of twenty microsatellite loci in Japanese sea cucumber (Stichopus japonicus). Mar. Biotechnol. 7(3): 179-183
In: Marine Biotechnology. Springer-Verlag: New York. ISSN 1436-2228, more
Peer reviewed article  

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Keywords
    Genetic variability; Sea cucumbers; Apostichopus japonicus (Selenka, 1867) [WoRMS]; Marine

Authors  Top 
  • Kanno, M.
  • Li, Q.
  • Kijima, A.

Abstract
    Twenty microsatellite markers were first developed from the Japanese sea cucumber Stichopus japonicus using an enrichment protocol. Of the 20 microsatellite loci, 19 loci were polymorphic in the population examined. At these polymorphic loci, the number of alleles per locus varied from 2 to 15, and the observed heterozygosities ranged from 0.03 to 0.97, which is considerably higher than those previously found for allozymes. The high variability of the microsatellite markers identified in this study will make them excellent tools for genetic analyses of S. japonicus.

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