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Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina)
de Swart, R.L.; Kluten, R.M.G.; Huizing, C.J.; Vedder, L.J.; Reijnders, P.J.H.; Visser, I.K.G.; Uytdehaag, F.G.C.M.; Osterhaus, A.D.M.E. (1993). Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina), in: de Swart, R.L. (1995). Impaired immunity in seals exposed to bioaccumulated environmental contaminants = Immuunsuppressie in zeehonden blootgesteld aan in de voedselketen geaccumuleerde milieuvervuilende stoffen. pp. 23-34
In: de Swart, R.L. (1995). Impaired immunity in seals exposed to bioaccumulated environmental contaminants = Immuunsuppressie in zeehonden blootgesteld aan in de voedselketen geaccumuleerde milieuvervuilende stoffen. PhD Thesis. Erasmus Universiteit Rotterdam: Rotterdam. ISBN 90-9008419-3. 140 pp., more

Also published as
  • de Swart, R.L.; Kluten, R.M.G.; Huizing, C.J.; Vedder, L.J.; Reijnders, P.J.H.; Visser, I.K.G.; Uytdehaag, F.G.C.M.; Osterhaus, A.D.M.E. (1993). Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (Phoca vitulina). Vet. Immunol. Immunopathol. 37(4): 217-230, more

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Keywords
    Antigens; Blood cells; Immunology; Induction; Induction; Induction; Lymphocytes; Marine mammals; Mitogens; Response analysis; Phoca vitulina Linnaeus, 1758 [WoRMS]; Marine

Authors  Top 
  • de Swart, R.L.
  • Kluten, R.M.G.
  • Huizing, C.J.
  • Vedder, L.J.
  • Reijnders, P.J.H., more
  • Visser, I.K.G.
  • Uytdehaag, F.G.C.M.
  • Osterhaus, A.D.M.E.

Abstract
    In vitro assays were developed for studies concerning the functioning of the immune system of the harbour seal (Phoca vitulina). Proliferative responses of peripheral blood mononuclear cells (PBMC) were measured after stimulation with different concentrations of the mitogens concanavalin A (Con A), pokeweed mitogen (PWM), phytohaemagglutinin (PHA) or lipopolysaccharide from Salmonella typhimurium (LPS). Con A and PWM induced strong proliferative responses, which PHA and LPS induced comparatively low proliferative responses. Antigen-specific immune responses were measured after immunization of seals with an inactivated rabies vaccine and/or with tetanus toxoid. Antigen-specific proliferation of PBMC and the presence of antigen-specific antibody forming cells were demonstrated for both antigen s in the PBMC of immunized animals. The responses measured in vitro correlated well with the development of specific serum antibody titers to these antigens.

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