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Some methods for study of the biochemical constitution of marine invertebrates
Giese, A.C. (1967). Some methods for study of the biochemical constitution of marine invertebrates. Oceanogr. Mar. Biol. Ann. Rev. 5: 159-186
In: Oceanography and Marine Biology: An Annual Review. Aberdeen University Press/Allen & Unwin: London. ISSN 0078-3218, more
Peer reviewed article  

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Document type: Review

    Biochemical composition; Marine invertebrates; Methodology; Marine

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  • Giese, A.C.

    At a time when interest is growing in the biochemical analysis of marine invertebrates, especially their content of macromolecules (protein, nucleic ocids, carbohydrates) and reserves (lipids, carbohydrates, non-protein nitrogenous compounds (NPN)), a review of the scattered literature makes it evident that diverse methods for the determination of these chemical constituents are being employed. Since values for a chemical constituent in the same tissue of a given species may depend upon the method employed, a comparison and reconsideration of some of the methods, both preparative and biochemical, used in such studies would appear useful. Some of the points considered here have been raised individually in other papers, but they have not been brought to a focus, which is the purpose of this paper. Before considering the methods, however, it is desirable to consider the choice of materials for analysis because investigators have chosen their materials in widely different ways. The results of the author and his associates will be frequently quoted to illustrate the problems involved in this kind of work. It is recognized that the present account falls short of completeness inasmuch as many pertinent topics are not covered and others are covered only from the standpoint of our rather limited experience. It might therefore be taken as the starting point for other reviews covering topics not considered here, or covering more completely those introduced. If it stimulates others to do this it will have served its purpose.

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