|Biochemical and molecular effects of perfluorooctane sulfonate (PFOS) exposure in European sea bass (Dicentrarchus labrax)|
Van de Vijver, K.I.; Soetaert, A.; Moens, L.; Van der Ven, K.; van Remortel, P.; Maras, M.; De Coen, W.M. (2006). Biochemical and molecular effects of perfluorooctane sulfonate (PFOS) exposure in European sea bass (Dicentrarchus labrax), in: Van de Vijver, K.I. Blootstelling en effectevaluatie van perfluorverbindingen op mariene en estuariene organismen = Exposure and effect assessment of perfluorinated alkylated substances in marine and estuarine organisms. pp. 149-174
In: Van de Vijver, K.I. (2006). Blootstelling en effectevaluatie van perfluorverbindingen op mariene en estuariene organismen = Exposure and effect assessment of perfluorinated alkylated substances in marine and estuarine organisms. PhD Thesis. Universiteit Antwerpen: Antwerpen. 206 pp., more
Fluorinated hydrocarbons; Gene expression; Genetic abnormalities; Dicentrarchus labrax (Linnaeus, 1758) [WoRMS]; Marine
|Authors|| || Top |
- Van de Vijver, K.I., more
- Soetaert, A., more
- Moens, L., more
- Van der Ven, K.
- van Remortel, P.
- Maras, M., more
- De Coen, W.M., more
Toxic effects of perfluorinated chemicals, and perfluorooctane sulfonic acid (PFOS) in particular, have mainly been studied in mammalian model species. Information on the impact of these chemicals on marine ecosystems is rather scarce. The aim of this study was to elucidate the toxicological mode of action of PFOS in the European Sea bass (Dicentrarchus labrax) using selected biochemical endpoints (e.g. serum alanine aminotransferase activity (ALT), serum electrolyte levels and hematocrit) and molecular responses (using cDNA microarrays). Juvenile fish were exposed to four different PFOS concentrations (0.1; 1; 10 and 100 mg/l) via water and after 14 days effects were assessed in liver and serum. The liver PFOS concentration was positively and significantly related to both ALT activity and total serum protein content, and significant negative relations were found with the serum Na+ and K+ concentrations and the hematocrit values. The microarrays used in this study were constructed with cDNA libraries obtained from Suppression Subtractive Hybridisation (SSH) PCR experiments. Several genes were identified to be PFOS responsive. Major functional classes of differentially expressed genes included detoxification-related genes, oxidative stress related genes, genes involved in energy or iron/heme metabolism, genes which play a role in the immune system, translation and/or transcription processes and signal transduction. Real-Time PCR confirmed the differential expression of selected genes.