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Protein carbonylation and heat shock response in Ruditapes decussatus following p,p'-dichlorodiphenyldichloroethylene (DDE) exposure: a proteomic approach reveals that DDE causes oxidative stress
Dowling, V.; Hoarau, P.C.; Romeo, M.; O’Halloran, J.; Van Pelt, F.; O’Brien, N.; Sheehan, D. (2006). Protein carbonylation and heat shock response in Ruditapes decussatus following p,p'-dichlorodiphenyldichloroethylene (DDE) exposure: a proteomic approach reveals that DDE causes oxidative stress. Aquat. Toxicol. 77(1): 11-18. http://dx.doi.org/10.1016/j.aquatox.2005.10.011
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X, more
Peer reviewed article  

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Keywords
    DDT; Electrophoresis; Heat shock; Proteins; Ruditapes decussatus (Linnaeus, 1758) [WoRMS]; Marine
Author keywords
    DDE; DDT; stress response proteins; proteomics; Tapes semidecussatus;Ruditapes decussatus; oxidative stress; protein carbonylation;organochlorine; organochloride; heat shock responsc; hsp60; hsp70; hsp90

Authors  Top 
  • Dowling, V.
  • Hoarau, P.C.
  • Romeo, M.
  • O’Halloran, J.
  • Van Pelt, F.
  • O’Brien, N.
  • Sheehan, D., correspondent

Abstract
    Protein carbonylation and levels of heat shock proteins (hsp; 60, 70 and 90 kDa) were measured in gill, mantle and digestive gland of Ruditapes decussatus following exposure to p,p′-dichlorodiphenyldichloroethylene (DDE). Heat shock response was measured by immunoblotting using antibodies specific to heat shock proteins (hsps). Densitometry analysis of individual bands revealed no difference between control and treated samples except appearance of hsp90 in DDE-treated mantle. Carbonylated protein content was determined following 2,4-dinitrophenylhydrazine derivatization and two-dimensional electrophoresis coupled with western blotting. Immunoblotting with dinitrophenol-specific antibody revealed extensive differences in both extent and number of carbonylated proteins in mantle and digestive gland in response to DDE while gill was unaffected. These results demonstrate for the first time that DDE causes tissue-specific formation of reactive oxygen species in clams.

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