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Methodical problems in the measurement of phytoplankton ingestion rate by gut fluorescence
Baars, M.A.; Helling, G.R. (1985). Methodical problems in the measurement of phytoplankton ingestion rate by gut fluorescence. Hydrobiol. Bull. 19(1): 81-88
In: Hydrobiological Bulletin. Netherlands Hydrobiological Society: Amsterdam. ISSN 0165-1404, more
Peer reviewed article  

Available in  Authors 

    Fluorescence; Food intake; Food intake; Grazing; Gutting; Ingestion; Measurement; Phytoplankton; Zooplankton; Copepoda [WoRMS]; Marine

Authors  Top 
  • Baars, M.A.
  • Helling, G.R.

    Due to its simplicity and applicability under field conditions the gut fluorescence method is becoming popular in studies of grazing on phytoplankton by copepods. The variety of the problems in processing of samples and in procedure of calculation is discussed, and it is concluded that aspects as influence of freezing and background fluorescence deserve more study. The relation between the measurement of gut clearance rate in starvation experiments and the actual get passage time under field conditions is treated in a theoretical model. Changes in the pattern of faecal pellet production result in overestimating gut passage time and may invalidate the general applicability of the model of exponential decrease of gut contents. In a starvation experiment with Calanus finmarchicus coprophagy by CIII/CIV occurred within 2 h. The current gut fluorescence technique is not useful for detecting selective grazing but the application of HPLC analysis of algal pigments may open new possibilities.

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